Cannabis Sativa Seed Oil is derived from Hemp and used in skincare formulas to provide essential moisture and nourishment while helping strengthen skin’s barrier. Since it is derived from the stalks and seeds of the plant, Cannabis Sativa Seed Oil contains less than 0.3% THC making it non-psychoactive.
Cannabinoids are active compounds produced in the Cannabis sativa plant and are generally concentrated in the plant’s leaves. Two of the most well-known Cannabinoids are THC and CBD.
Cannabis Sativa Seed Oil
THC, or Tetrahydrocannabinol, is a cannabinoid and the primary psychoactive component found in Marijuana. Marijuana typically averages 10% THC—well above the amount found in Hemp.
Blank matrix was obtained as described in our previous work (Citti et al., 2018c). Briefly, 22 g of hemp seeds (cleared of bracts) were washed with ethyl alcohol 96% (3 × 100 mL) in order to remove cannabinoids. Subsequently, the seeds were cold squeezed to obtain 4 mL of hemp seed oil where the level of cannabinoids was below the limit of detection. The final blank matrix (20 mL) was obtained by diluting the oil with 16 mL of 2-propanol.
Preparation of Standard Solutions and Hemp Seed Oil Samples
One-way ANOVA test of the ten hemp seed oil samples. Red points indicate statistically significant features, green points indicate features that do not contribute to the statistical difference (adjusted p-value cut-off: 0.01, post hoc test: Tukey’s Honest Significant Difference test).
Stock solutions of CBDV, CBDA, CBGA, CBG, CBD, CBN, Δ 9 -THC, Δ 8 -THC, CBC and THCA (1000 μg/mL) in methanol were diluted in blank matrix to the final concentration of 10 μg/mL. An aliquot of 100 μL of each sample was diluted with 890 μL of blank matrix and 10 μL of IS (Δ 9 -THC-d3 and CBD-d3, 200 μg/mL) to the final concentration of 1 μg/mL for CBDV, CBDA, CBGA, CBG, CBD, CBN, Δ 9 -THC, Δ 8 -THC, CBC and THCA and 2 μg/mL for IS.